TUDIES ON PRODUCTION OF POLY (4-HYDROXYBUTYRIC ACID) BY RECOMBINANT STRAIN OF ESCHERICfflA COLI
- VernacularTitle:利用重组大肠杆菌产聚-4-羟基丁酸的研究
- Author:
Shuishan SONG
- Publication Type:Journal Article
- Keywords:
Poly (4-hdroxybutyric acid), 4-hydroxybutyric acid, Recombinant E. coll, Polyester
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
A recombinant E. coli XL1-Blue (pKSSE5.3) habouring PHA sythase gene (phaC) of Ralstonia eu-tropha H16 and 4-hydroxybutyric acid: CoA transferase gene (orfZ) of Clostridium kluyveri was used to produce poly (4-hydroxybutyric acid), P (4HB), homopoiyester in M9 salts medium with glucose and 4-hydroxybutyric acid as carbon sources. Optimization of medium type and cultivation conditions and fed-batch culture were carried out. The results showed that the final cell dry weight, P (4HB) concentration and P (4HB) content were 13 g/L, 5g/L, and 36%, respectively in a 27L stirred and aerated fermentor after 68 hours of fed-batch culture. A large amount of P (4HB) was isolated by extraction of lyophilized cells with chloroform and make it possible to further examine the material properties and applications of P (4HB) homopoiyester from recombinant E. coli.
