Comparison of single factor design and response surface design to optimize the reaction system for randomly amplified polymorphic DNA
- VernacularTitle:随机扩增多态性DNA反应体系优化设计方案的比较
- Author:
Mingliang YE
;
Quanli WANG
;
Liangping HU
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Random amplified polymorphic DNA technique;
Factor analysis, statistical;
Research design
- From:
Chinese Journal of Laboratory Medicine
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain optimum scheme on reaction system for randomly amplified polymorphic DNA(RAPD) of Pseudomonas aeruginosa. Methods The optimum reaction systems of RAPD were obtained by single factor design and response surface design(RSD). Results The multiple optimum reaction systems caused by effect of interaction on multifactor were observed in single factor design, and the concentrations of key components of the optimum reaction system with good stability obtained by response surface design were 2.0 mmol/L of Mg 2+ , 0.5 ?mol/L of primer and 0.5 ng/?l of template. Conclusion To optimize the reaction system of RAPD, RSD is simpler, more scientific, and more reasonable than single factor design.
