Topoisomerase gene mutation and mechanism of ureaplasma urealyticum resistant to quinolones
- VernacularTitle:解脲脲原体拓扑异构酶基因突变与耐喹诺酮类药物关系的研究
- Author:
Wenbo ZHANG
;
Yimou WU
;
Weiguo YIN
- Publication Type:Journal Article
- Keywords:
Ureaplasma urealyticum;
Gene, structural;
Mutation;
Drug resistance, microbial;
Quinolones
- From:
Chinese Journal of Laboratory Medicine
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relation of type Ⅱ topoisomerase gene mutation and mechanism of Ureaplasma urealyticum resistant to quinolones. Methods 13 isolates of Uu resistant to 6 quinolones were selected from 184 clinical isolates by using broth dilution method, and their gyrA, gyrB, parC, parE were amplified by PCR.After sequencing, results were compared with the nucleotide sequence of susceptible reference strain. ResultsMICs of resistant Uu isolates were 4 to 32 fold higher than susceptible reference strain counter parts; sequence comparison revealed a C to A change at 87nt of gyrA QRDR led to the substitution of aspartic acid by glutamic acid and a C to T change at 50nt of parC QRDR led to the substitution of serine by leucine, with no amino acid change observed in GyrB and ParE. Conclusion These results suggest that a C to A change at 87nt of gyrA QRDR and a C to T change at 50nt of parC QRDR are associated with quinolone resistance of Uu.
