Reversal Effects of Tumor Necrosis Factor ? and Interferon ? on MDR in K562 Cell Line Resistant to Adriamycin
- VernacularTitle:肿瘤坏死因子?和干扰素?逆转K562/A02耐药性的研究
- Author:
Jie LI
;
Weijian GUO
;
Liangzhong XU
- Publication Type:Journal Article
- Keywords:
K562;
TNF-?;
IFN-?;
multidrug resistance;
reversal
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objetive: To observe the reversal effects of tumor necrosis factor (TNF) ? and interferon (IFN) ? on multidrug resistance (MDR) in K562 cell line resistant to adriamycin (ADR) (K562/A02). Methods: After treatment with TNF-? and IFN-? respectively, K562/A02 sensitivity to ADR was investigated using tetrazolium dye assay. MDR1 gene expression was assayed by semiquantitative reverse transcription-polymerase chain reaction and immunocytochemistry staining. Intracellular ADR concentration was also observed with flow cytometry. Results: The reversal activity after treatment with TNF-? or IFN-? was found to be increased up to 6 and 5-fold respectively at 24 h, and the peak with the increase of 10 and 8-fold respectively was seen at the 48 h (both P
