Isolation of Peptides Binding to KDR Specifically Using Phage Display
- VernacularTitle:筛选与KDR有结合活性的小肽
- Author:
Jie WU
;
Huipeng CHEN
;
Hongbin ZHANG
;
Jie WANG
;
Wenling ZHENG
- Publication Type:Journal Article
- Keywords:
KDR;
lead drug;
peptide library
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To isolate small molecular polypeptides which bind to KDR specifically using C7 and 12 peptide libraries. Methods: KDR/IgGFc was coated directly on plates, C7 and 12 peptide libraries are then applied, followed by vigorous washings in washing buffers to remove most non-specifically bound phage and to select specific phage particals by VEGF suspension and elution in acid buffers. The positive phage clones were detected by ELISA, cell-ELISA and competitive binding ELISA. Results: After three washes, 12 positive phage clones were selected and sequenced. Conclusion: A conserved peptide motif was not found in these sequences. The peptide binding to KDR specifically may be helpful for lead drug to improve selectivity and decrease the side effect of anti cancer drug in clinical treatment.
