Comparison of Serum-Free Medium AIMV with Standard Serum-Containing Medium in Culturing Cells Activated with IFN-?,IL-2 and Anti-CD3
- VernacularTitle:无血清培养基与完全培养基对体外诱导免疫细胞支持作用的比较
- Author:
Jing SUN
;
Hongsong CHEN
;
Yan GAO
;
Songxia WANG
;
Yi ZHANG
;
Yu WANG
- Publication Type:Journal Article
- Keywords:
serum-free medium;
immunotherapy;
phenotype;
cytokine;
cell culture
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare serum-free medium AIMV with standard serum-containing medium in culturing cells activated with IFN-?,IL-2 and anti-CD3. Methods: Peripheral blood mononuclear cells (PBMC) separated from donors were cultured with IFN-?,IL-2 and anti-CD3 in serum-free medium AIMV or in standard serum-containing medium separately. Proliferation, phenotypes and cytokine secretion of cells cultured in the two different media were compared. The inhibitory effects on hepatitis B virus replication in vivo were observed after transfusion of cells cultured in different media. Results: Compared with cells cultured in standard serum-containing medium, cells cultured in serum-free medium AIMV could proliferate well enough to be applied to immunotherapy; More percentage of cells cultured in AIMV expressed CD25; Cells cultured in serum-free medium secreted more IFN-?; After transfusion of cells cultured in AIMV, hepatitis B replication could be inhibited more evidently. Conclusion: Serum-free medium AIMV was better than serum-containing medium in culturing cells for immunotherapy.
