A new method detecting the aberrant IgA in serum for IgA nephropathy diagonosis
- VernacularTitle:血清异常IgA测定诊断IgA肾病的新方法
- Author:
Qiu YAN
;
Wenli ZHANG
;
Zhengmei ZHU
- Publication Type:Journal Article
- Keywords:
IgA nephritis;
Immunoglobulin;
Helia aspera lectin
- From:
Chinese Journal of Laboratory Medicine
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the patients with IgA nephropathy by glycobiology and immunological technique. Methods An helia aspera lectin ELISA immunoassay was developed to discriminate the aberrantly glycosylated IgA in serum from normal IgA molecules by using HAA lectin, which reacts preferentially with the GalNAc of the terminal sugar chain, and AKP conjugated specific IgA antibody. Results The results given by A 405 nm values from 156 controls, 22 patients with IgAN and 19 patients with uncertain IgAN were 0.22?0.10,0.68?0.17 and 0.38?0.15, respectively. The significance between the controls and the patients with IgAN was observed ( P
