Aedes albopictus: Cloning and Identification of the Acetylcholinesterase Gene Fragment from the Mosquito
- VernacularTitle:白纹伊蚊乙酰胆碱酯酶基因片段克隆及鉴定
- Author:
Mingwei WU
;
Lingmin ZHANG
;
Jionglie HUANG
;
Guoli ZHOU
;
Yu WU
;
Shuangxing ZHAO
- Publication Type:Journal Article
- Keywords:
Aedes albopictus, acetylcholinesterase, insecticide resistance, clone.
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate, clone and identify the acetylcholinesterase (AChE) fragment from the mosquito, Aedes albopictus, in relation to exploring mechanism of insecticide resistance. \ Methods\ The genome DNA extracted from the mosquito was used for degenerate polymerase chain reaction (PCR) and the two pairs of oligonucleotides encoding the highly conserved protein sequences were used as primers. The reaction products were cloned to T\|vector and transfected into E\^coli JM 109. The replicative form DNA of recombinant vector extracted from E\^coli JM 109 through alkalilysis was identified by the methods of digestion with EcoRⅠand SalⅠ and PCR. \ Results\ The products of degenerate primers polymerase chain reaction were obtained and the identified clone belongs to the AChE fragment of the mosquito.\ Conclusion\ The clone was identified as the AChE fragment of Aedes albopictus.
