Study on concentration of docosahexenoic acid in glyceride by hydrolysis of fish oil withlipases
- VernacularTitle:脂肪酶催化水解法浓缩鱼油DHA甘油酯的研究
- Author:
Hongqi SHI
;
Jinlai MIAO
;
Guangyou LI
;
Fayi LIU
;
Chengfan LIU
- Publication Type:Journal Article
- Keywords:
docosahexenoic acid glyceride;
Candida lipalytica Lipase;
hydrolysis;
concentration;
fish oil
- From:
Chinese Journal of Marine Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In this paper,the method about hydrolysis of fish oil with lipases for concentrating DHA(docosahexenoic acid) in glycerides was studied.Six lipases were screened with comparing the activities of hydrolysis of fish oil.Candida lipalytica Lipase is resistant to generating DHA in fish oil.The Effects of the quantity of lipases,the emulgent,hydrolysis time,temperature in the process,the ratio of oil to water were studied.The optimum conditions for the hydrolysis of fish oil are 12 hours of hydrolysis under 45℃,with 300 u?g -1 .The emulgent was and the ratio of oil to water was 0.4 mL?g -1 .Under these conditions,the content of DHA in glyceride were concentrated from 18.7% to 34.0%.But the EPA content remained close to that of fish oil.The DHA glyceride containing triglyceride,diglyceride and monoglyceride was identified by IR spectrum combining with separation of HPLC.
