The relationship between the cyclin kinase inhibitor p27 and the hypertrophy of mesangial cell cultured in high glucose
- VernacularTitle:高糖培养的系膜细胞肥大与周期素激酶抑制剂p27的关系
- Author:
Xiaobin MEI
;
Ruolan CUI
;
Weijie YUAN
- Publication Type:Journal Article
- Keywords:
Cell cycle regulatory protein Cyclin kinase inhibitor p27 Diabetic nephropathy
- From:Chinese Journal of Diabetes
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Our study aimed at the role of p27 on the hypertrophy of mesangial cell (MC) cultured in high glucose. Methods The p27 protein of MC lysate was detected with western blotting analysis. The degree of cultured MC hypertrophy was estimated through [3H]-thymidine incorporation and [3H]-leucine incorporation. The effect of reducing p27 expression on cell hypertrophy was analysed with p27 antisense oligodeoxynucleotide (ODN) phosphorothioate. Results in MC cultured in high glucose (450mg/dl) serum-free DMEM compared with MC cultured in normal glucose (100mg/dl) serum-free DMEM, p27 increased, [3H]-leucine incorporation increased and[3H]-thymidine incorporation decreased;p27 antisense ODN transfection reduced [3H]leucine incorporation, increased [3H] thymidine incorporation of MC cultured in high glucose senumfree DMEM. Increasing medium osmolarity with D-mannitol failed to induce p27 expression of MC. Conclusion p27 protein increased in MC cultured in high glucose. High level of p27 played an important role in MC hypertrophy induced by high glucose. Because the cell cycle is controlled by the interaction between the positive cell cycle regulatory proteins(CCRP) and negative CCRP, further research is needed to study the expression of the positive and negative CCRP in MC in order to better understand the role of CCRP in MC hypertrophy.
