Genotyping of human platelet antigen system 5 by PCR restriction fragment length polymorphism
- VernacularTitle:限制性片段长度多态性分析人血小板抗原5系统基因型
- Author:
Zhenyu LU
;
Dazhuang LIU
;
Ziyan ZHU
- Publication Type:Journal Article
- Keywords:
Genotypes;
Polymerase chain reaction;
Antigens, human platelet;
Polymorphism, restriction fragment length
- From:
Chinese Journal of Laboratory Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) method using designed primers for determining the genotype of humen platelet antigens (HPA)5 system. Methods HPA 5 system of 25 healthy blood donors were genotyped using PCR RFLP method. The results obtained by PCR RFLP were compared with those determined by allele specific oligonucleotid hybridization (PCR ASO). Results The results of HPA 5 system obtained by PCR RFLP in 25 health donors were as follows: 24 of aa, 1 of ab and 0 of bb. All were in good agreement with those determined by PCR ASO. Conclusions Because PCR RFLP method is plain, fast and reliable for HPA 5 system genotyping, it is suitable for the diagnosis and therapy of neonatal alloimmune thrombocytopenia, posttransfusion purpura, platelet transfusion refractoriness and so on..
