Flow injection immunoassay for insulin using a new synergistic enhanced chemiluminescence
- VernacularTitle:流动注射协同增强化学发光免疫分析法测定胰岛素
- Author:
Jianxin LUO
;
Xiao ZHANG
;
Xiucen YANG
- Publication Type:Journal Article
- Keywords:
Flow injection analysis;
Immunity analysis;
Chemiluminescence;
Insulin
- From:
Chinese Journal of Laboratory Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a new non radioimmunoassay for detecting insulin. Methods We put together the techniques of chemiluminescent analysis, flow injection analysis and immunoassay and established a method,flow injection chemiluminescent immunoassay, for insulin determination using sandwhich procedure with the horseradish peroxdase (HRP) as label of antibody and using the detection system of synergistic enhanced chemiluminescence of para phenylpenol and sodium tetraphenylborate with HRP catalysed oxidation of luminol. Results Satisfactory results were obtained in the dynamic range 0.15～60.00 ?U with a relative coefficient of 0 997 2 ( P
