The polymorphism in hepatic nuclear factor-1? gene and associated plasma insulin concentration
- VernacularTitle:肝细胞核因子-1?基因多态性与血浆胰岛素水平的变化
- Author:
Zhengyi TANG
;
Guang NING
;
Reuming HU
- Publication Type:Journal Article
- Keywords:
Type 2 diabetes mellitus HNF-1? gene Polymorphism
- From:
Chinese Journal of Diabetes
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the role of the mild changes in hepatic nuclear factor (HNF)-1? gene in the pathogenesis of type 2 diabetes mellitus. Methods With PCR, denatured gradient gel electrophoresis and DNA sequencing,the genomic DNAs from the blood of type 2 diabetic patients and normal control were analyzed to find the changes in HNF-1? gene. These changes were added up in-groups and compared with plasma insulin concentration. Results Code 459 CTG changed to TTC,codes 489 AGC changed to AAC in the 7th exon;no 7G changed to A in the 7th intron. The 3 point changes were of one kind of polymorphism. Its frequency to normal genotype was 61: 39 in normal control, 56: 44 in type 2 diabetes mellitus. Homozygotes of the polymorphism in the group of type 2 diabetes mellitus had slightly lower plasma insulin concentration, but t-test result was P
