Serial Expression of Human ?,? globin Genes in Escherichia coli
- VernacularTitle:人?、?珠蛋白基因以串联形式在大肠杆菌中的表达
- Author:
Hao ZHANG
;
Quanli WANG
;
Qi FENG
- Publication Type:Journal Article
- Keywords:
Hemoglobin;
Escherichia coli;
Gene expression,?、? globin
- From:
Chinese Journal of Blood Transfusion
1988;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To make an attempt to express serialy the human ?,? globin in Escherichia Coli in order to carry out the research on blood substitute based on the genetic recombination of hemoglobin.Methods The routine molecular biology technology was used.Results The serial genetic fragment of ?,? globins containing the hemoglobin was amplified by PCR.The genetic sequencing showed that there was no unexpected mutation.And then,the serial genes were cloned into the pBV220 expression carrier.Undergoing thermal inducement,the expression product could reach about 20% of total bacterial protein.The expression product assumed the form of inclusion body,and it was vertified by Western Blotting.Conclusion This much can lay the solid foundations for further launching the research on expression and pruification of recombinant human hemoglobin genes.
