Expression of glycosylphosphatidylinositol-anchored protein in lymphocytes and the subsets of healthy individuals
- VernacularTitle:糖化肌醇磷脂结合蛋白在健康人淋巴细胞及其亚群的表达
- Author:
Wei CUI
;
Min YANG
;
Zhinan ZHANG
- Publication Type:Journal Article
- Keywords:
Lymphocyte subset;
Glycosylphosphatidylinositols;
Carrier proteins;
Healthy control
- From:
Chinese Journal of Laboratory Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of glycosylphosphatidylinositol(GPI) anchored protein in lymphocyte subsets of healthy individuals. Methods Flow cytometry and two/three color McAbs were used to detect CD59 expression in granulocytes, lymphocytes and subsets. Results In 50 samples, granulocytes mainly showed a single population of cells strongly positive for CD59, the percentage of CD59 expression was (98.6?1 5)%. Lymphocytes had a small population of weakly positive and negative for CD59, positive cells being (90 0? 14.9)%. CD3 +T cells had a similar profile as that of total lymphocytes, but there were more weakly positive /negative cells and less strong positive cells. As compared with CD3 +T cells, CD4 +T cells had a larger population of strongly positive for CD59, positive cells being (92.9?8.1)%; while CD8 +T cells had a smaller population of strongly positive, positive cells being (74.6?9.1)%. CD59 expression of activated T cells was between that of CD3 +and CD8 +T cells. CD45RA +and CD45RO +T cells both had a similar profile like that of CD3 +T cells. CD20 +B cells showed a similar profile to that of granulocytes, positive cells being (96.3?0 6)%. Conclusions Population of low or negative expression of CD59 among the lymphocytes of healthy controls was related to T cells, especially CD8 +T cells. B cells had uniform positive expression of CD59, and could be a candidate for detecting PNH.
