Rapid detection of Escherichiacoli with test paper of Na p-nitrophenyl ?-D-Glucuronate
- VernacularTitle:对-硝基苯葡糖苷酸钠纸片法快速检测大肠埃希菌
- Author:
Jiali SUN
;
Sha MI
;
Xiaolan LIU
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
Culture media;
? glucuronidase
- From:
Chinese Journal of Laboratory Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a new method for rapid detection of E.Coli.with a new substrate Na p nitrophenyl ? D glucuronate (PNP Glucu Na). Method The test paper was immersed with of Na p nitrophenyl ? D glucuronate which was synthesized by us and another paper with culture medium and phorphate buffer.Colour change was observed to detect the E.coli. Results An E.coli concerntration of 4?10 7 took 0.5 hour, while a concentration of 4?10 0 took 12 hours. 138 strains of E.coli and 190 strains of other bacteria were examined . The sensitivity was 94.2% and the specificity was 92.6%. Conclusion This method is rapid, accurate, simple, and economical for detecting E.coli.
