The influence of pretreatment with Anti-B lymphocyte serum on immunogenecity of islet preparations
- VernacularTitle:抗体预处理对胰岛移植物的影响
- Author:
Weiping DONG
;
Hongde ZHANG
;
Yufei WANG
- Publication Type:Journal Article
- Keywords:
Islet Graft Pretreatment
- From:
Chinese Journal of Diabetes
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of pretreatment with anti-B lymphocyte serum on the immunogenecity and endocrine function of islet preparations.Methods Porcine ICCs were pretreated with anti-B lymphocyte serum which was produced using the mixed adult porcine B lymphocyte as antigen.The immunogenecity of islet was measured by mixed islet lymphocyte culture (MILC) and SLA-DR positive cell number within the islet and the islet function was assayed with insulin release test.Results The cpm value of MILC of the group pretreated with anti-B lymphocyte serum and the MHCⅠ antigen and SLA-DR antigen positive cell numbers was markedly decreased in comparison with that of control group ( P
