CpG-ODN Enhances Anti-Tumor Effects of Non-Replicating Vaccinia Virus-Modeified Oncolysates
- VernacularTitle:CpG-ODN可增强痘苗病毒修饰的癌溶物的抗肿瘤效果
- Author:
Fei GUO
;
Honglin XU
;
Roujian LU
;
Jun LI
;
Li RUAN
- Publication Type:Journal Article
- Keywords:
CpG-ODN;
vaccinia virus;
tumor;
immunotherapy
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the antitumor effect of CpG-ODN combining non-replicating recombinant vaccinia virus in tumor immunotherapy.Methods: CpG-ODN and constructed vaccinia virus v△11?75 were combined to study the antitumor effects in the walker′s rat tumor model.Results: Recombinant vaccinia virus v△11?75 lost the replicating capacity on human cell line,143TK - cell.The genes between HindⅢ C & K fragment of vaccinia virus TianTan strain were deleted,verified with Southern-blot. In the Walker′s tumor model of Wistar rats,Combinational immunotherapy with CpG-ODN and non-replicating vaccinia virus-modified WRC256 oncolysates resulted in prolonged life span and reduced tumor hyperplasia. Conclusion: CpG-ODN can enhance the antitumor effects of non-replicating vaccinia virus-modified oncolysates,providing a new route of tumor therapy.
