Recombinant Interferon ?2a Synergistically Enhances SKOV3 Ovarian Tumor Cell Killing by the HSV-tk/GCV System
- VernacularTitle:重组IFN-?2a与HSV-tk/GCV系统协同作用增强对于卵巢癌细胞系SKOV3杀伤作用的体外研究
- Author:
Yuejuan CHENG
;
Lingya PAN
;
Yi ZHANG
- Publication Type:Journal Article
- Keywords:
ovarian cancer;
HSV-tk/GCV;
IFN-?2a
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine if IFN-?2a and HSV-tk/GCV have synergy on the cell killing of the SKOV3 human ovarian tumor cell line. Methods: hytk gene retroviral vector plasmid was transduced into SKOV3 cells. Both the cytotoxicity and the bystander effect were evaluated after the treatment of GCV. IFN-?2a and HSV-tk/GCV were used together to determine if they have synergy on the tumor cell killing effect, then post-treatment cell cycle analysis was carried out.Results: A dose dependent cell killing of SKOV3/hytk was observed after treatment with GCV. Bystander effect was seen in mixed culture of hytk-positive and -negative cells. Furthermore, IFN-?2a had a significant (P
