PCR-molecular beacon assay for detecting Mycobacterium tuberculosis in clinical specimens
- VernacularTitle:聚合酶链反应分子灯塔法检测结核分枝杆菌
- Author:
Guoli LI
;
Ming ZHAO
;
Yuhui ZHUANG
;
Al ET
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Polymerase chain reaction;
Fluorogenic probe hybridzation
- From:
Chinese Journal of Laboratory Medicine
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the value of PCR molecular beacon assay. In detecting mycobacterium tuberculosis in clinical specimens. Methods The sputum specimens of 142 patients with pulmonary tuberculosis and 42 patients with respiratory system diseases other than tuberculosis were tested by smears, cultures and PCR molecular beacon assays. Results The sensitivities of smears. cultures and molecular beacon assay in detecting mycobacterium tuberculosis in sputum specimens of patients with pulmonary tuberulosis were 35.2%,35.9% and 44. 4%, respectively. The specificity of molecular beacon assay in testing clinical specimens was 100%. Conclusion PCR molecular beacon assay is in a hermetially sealed simple tube, rapid and anticontaminative. This assay is higher in sensitivity and specifity,than smears and cultures is one of the effective mothods for diagnosis of tuberculosis.
