SEQUENCE ANALYSIS OF SSU rDNA VARIABLE REGIONS OF LEISHMANIAISOLATES FROM HILLY FOCI AND PLAIN FOCI OF CHINA
- VernacularTitle:我国内脏利什曼病山丘疫区与平原疫区利什曼原虫SSUrDNA多变区序列分析
- Author:
Lingyi BU
;
Xiaosu HU
;
Baoqian JING
;
Taoli YI
- Publication Type:Journal Article
- Keywords:
Leishmania, small subunit ribosomal DNA, polymerase chain reaction, cloning, sequence analysis, genetic variation
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective] To analyze the sequence difference of the SSU rDNA variable regions of Leishmania isolates from hilly foci and plain foci of China. [Methods] Specific SSU rDNA fragments from nuclear DNA of five Leishmania species and isolates were amplified by PCR. The amplified DNA fragments were cloned into pGEM R\|T Easy vector. The specific fragments were sequenced by the automated DNA sequencer. [Results] Sequence analysis showed that the amplified DNA fragments of five Leishmania species and isolates were all 392 bp in length, point mutations were located in the two unique sequence (UQ\|Ⅰ and UQ\|Ⅱ); L.d.SC10 and L.d.GS7 had two same point mutations in UQ\|Ⅱ, only L.d.GS7 had one in UQ\|Ⅰ; no insertion/deletion. [Conclusion] Sequence difference of the SSU rDNA variable region existed between Leishmania isolates from hilly foci and plain foci; The sequences of the SSU rDNA variable regions of L.d. SD2 isolate and L.infantum were identical.
