ON MOLECULAR IDENTIFICATION AND TAXONOMIC STATUS OF ANOPHELES LESTERI AND ANOPHELES ANTHROPOPHAGUS IN CHINA (DIPTERA:CULICIDAE)
- VernacularTitle:我国雷氏按蚊和嗜人按蚊的分子鉴别和分类地位的探讨
- Author:
Yajun MA
;
Fengyi QU
;
Yucun CAO
;
Baoji YANG
- Publication Type:Journal Article
- Keywords:
Anopheles lesteri, Anopheles anthropophagus, molecular identification
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective] To clarify the taxonomic status of Anopheles lesteri and An.anthropophagus in China. [Methods] Using molecular identification (PCR assay and rDNA\|ITS2 sequencing) to examine the field anopheline mosquito specimens from Liaoning and Shandong. According to the ITS2 sequences, molecular phylogenetic tree was made. [Results] According to the molecular identification, An.lesteri and An.anthropophagus were distributed both in Liaoning Province and Shandong Province. The length and GC content of rDNA\|ITS2 sequence were 451 bp, 46 2% in An.lesteri (n=6), and 448 bp, 46 0% in An.anthropophagus (n=10), respectively. The ITS2 sequences from presentation sites were same in An.lesteri, while the intraspecies difference in An.anthropophagus was 0 88%. The specific difference between An.lesteri and An.anthropophagus was 25 7%. By analyzing molecular phylogenetic tree, the relationship between An.lesteri and An.sinensis, An.anthropophagus and An.liangshanensis was found to be closer. [Conclusion] According to the molecular identification, it was defined that An.lesteri and An.anthropophagus were sympatric independent species in China.
