Cloning and Screening of Mutant Human Hemoglobin Gene(?_(99) ,?_(82) Lys→Cys)
- VernacularTitle:突变型血红蛋白基因(?_(99),?_(82)Lys→Cys)的克隆和筛选
- Author:
Hao ZHANG
;
Quanli WANG
;
Qi FENG
- Publication Type:Journal Article
- Keywords:
Site directed;
Hemoglobin;
Gene;
Cloning
- From:
Chinese Journal of Blood Transfusion
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To mutate the codons which code the lysine at loci ? 99 ,? 82 in human hemoglobin gene to the codons which code the cystein.Methods:The objective gene to be mutated was cloned into pAlter Ex2 plasmis and then was mutated with the site directed mutagenesis mediated by oligonucleotide.Results:The sequencing of clones obtained by preliminary screening showed that the anticipated results were reached without any unexpected mutation.Conclusion:The results lay a foundation for the further development of research on expression and purification of recombinated mutant human hemoglobin gene.
