Comparison of the binding characteristics of insulin receptors on ovary, adrenal and hepatic plasma membrane from rats
- VernacularTitle:大鼠肝脏、卵巢及肾上腺胰岛素受体结合性质的比较
- Author:
Guilin LI
;
Suiqi GUI
;
Yuehua TANG
- Publication Type:Journal Article
- Keywords:
Insulin receptor Radioligand binding assay
- From:
Chinese Journal of Diabetes
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the binding characteristics of insulin receptors (IR) on ovary,adrenal and hepatic plasma membrane from rats. Methods The number and affinity of IR was detected by radioligand binding assay. Results Scatchard plot analyses showed that the receptor numbers for high affinity sites are 7. 359?10/mg protein, 8. 029? 10/mg protein and 6. 440?10/mg protein of liver,ovarian and adrenal plasma membrane with KD of 6.147?107 M-1, 1. 528?10, M-1, and 1. 010?107 M-1 that. The receptor numbers for low affinity sites are 2. 403?10/mg protein,2. 212?10/mg protein,and 2. 257?10/mg protein respectively with KD of 2. 920?10M-1,2. 008?10 M-1 and 0. 433?10sM-1. Conclusion There are abundent IR on ovary and adrenal just as many as those on liver, although the KD are smaller. It suggests that insulin may play an important regulatory role in ovary and adrenal.
