Detection of extended-spectrum-?-lactamases with VITEK-AMS and double-disk synergy test
- VernacularTitle:仪器法与纸片协同法检测超广谱?-内酰胺酶的结果比较
- Author:
Tieli ZHOU
;
Meijie DAI
;
Shengying CHI
- Publication Type:Journal Article
- Keywords:
beta lactamases;
?Escheri chia coli;
?Klebsiella pneumoniae
- From:
Chinese Journal of Laboratory Medicine
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To investigate the reliability of VITEK AMS for the detection of strains of E.coil and K.pneumoniae that produce ESBLs, and to determine the prevalence in our hospital of the two strains producing ESBLs.Method VITEK GNS 506 was used to detect ESBLs, and was compared with double disk synergy test. Results Among 48 strains of K.pneumoniase detected using VITEK GNS 506, 24 strains were found to be ESBLs positive, and 24 strains ESBLs negative. The result was consistent with that of double disk synergy test. Among 102 strains of E.coli detected using VITEK GNS 506, 41 strains were found to be ESBLs positive. The result was consistent with that of double disk synergy tests. 61 strains were found to be ESBLs negative detected using VITEK GNS 506. Among them, 19 strains were found to be ESBLs positive.Conclusion Detection ESBLs using VITEK AMS has some limitations in our area. Simple and reliable method should be used more with indicates for detection of ESBLs suitable for routine screening.
