ISOLATION OF ROSMARINIC ACID FROM CALLUS OF PERILLA FRUTESCENS AND STUDIES OF ITS INHIBITION ON THE GROWTHS OF BACTERIA AND FUNGAL
- VernacularTitle:紫苏愈伤组织迷迭香酸的纯化及抗菌活性研究
- Author:
Ronggui LI
;
Dewei TENG
;
Guicai DU
;
Bin WANG
- Publication Type:Journal Article
- Keywords:
Perilla frutescens, Callus, Rosmarinic acid, Antifugal and antibacterial activiti
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Perilla frutescens callus were induced from leave explants on MS medium suplemented with NAA and 2,4-D. The Rosmarinic acid (RosA) content of dried callus was 0.85%. The RosA was extracted from the callus with 80% alcohol and purified through extraction with ethyl acetate and a Sephadex LH-20 column chromatography. The parity of the final product was 95% as analyzed by HPLC RosA could inhibit the growths of Escherichia colt, Staphylococcus aueris and Rhizotonia with MICs of 300, 400, 800?g/mL, respectively.
