The Control of Apoptosis and Drug Resistance in Ovarian Cancer Cells Which Overexpressed bcl-2
- VernacularTitle:高表达bcl-2卵巢癌细胞系参与药物抵抗和细胞凋亡作用的研究
- Author:
Fang TINA
;
Chunhai LI
;
Chunhong YAN
;
Gaoming CHEN
- Publication Type:Journal Article
- Keywords:
bcl-2;
ovarian cancer;
drug resistance;
apoptosis
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate whether bcl-2 directly contributes to the development of drug resistance and apoptosis in o- varian cancer cell lines cells OC3. Methods: Retrovirus expression cector pLXSN-bcl-2 was constructed and was transfected to ovarian cancer cell line cells OC3 using Lipofectin,with empty vector pLXSN as a control. bcl-2 expession of transfected cells was analyzed by FACS and Western blot and Adr-induced cytotoxicity was measured by MTT assay. Apoptosis was also detected by PI DNA staining and DNA Ladder analysis.Results:pLXSN/bcl-2 transfected cells OC3/bcl-2 overexpressed bcl-2 and were resistant to Adr-induced cytotoxicity.The ability against Adr-induced apoptosis was increased. Conclusion: bcl-2 may play an important role in the resistance to Adr-inducing apoptosis, thereby increasing resistance of OC3/bcl-2 cells to chemotherapy.
