Mutagenesis of Transgenic Human Lung Adenocarcinoma Cell Line SPC-A-1/IL-2
- VernacularTitle:转基因细胞SPC-A-1/IL-2的致突变性研究
- Author:
Heshu LI
;
Dianjun LI
;
Lihua XIE
;
Xu LIU
;
Xuetao CAO
;
Shenglong YE
- Publication Type:Journal Article
- Keywords:
retrovirus;
cancer gene therapy;
interleukin-2;
Ames;
micronucleus
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the safety of transgenic human lung adenocarcinoma cell line SPC-A-1/IL-2 as tumor vaccine. Methods: IL-2 gene was introduced into human lung adenocarcinoma cell line SPC-A-1 and was expressed stably on the basis of the construction of retroviral packing cell line PA317/pLIL-2SN.The mutagenesis of both the DNA and supernatant of SPC-A-1/IL-2 in the and in vitro was tested by means of genetic toxicological techniques.Results:The result indicated that mutagenesis of both the DNA and the supernatant of transgenic cell SPC-A-1/IL-2 was not observed. Conclusion: The initial experiment suggested that the application of transgenic cell SPC-A-l/IL-2 as tumor vaccine was bisically safe and reliable.
