Reversion of Transforming Growth Factor ?_2 Mediated Immunosuppression in Lung Cancer by Icarrin
- VernacularTitle:淫羊藿甙逆转转化生长因子?_2免疫抑制作用的抗肿瘤研究
- Author:
Xiaoyan LI
;
Ling ZHANG
;
Yun WANG
;
Haiting MAO
;
Shulin CUI
- Publication Type:Journal Article
- Keywords:
ICA;
transforming growth factor ?2;
immunosuppression
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the influence of Icarrin(ICA) on transforming growth factor ?2(TGF?2) expression by PG cells and the mechanisms of reversion of TGF?2 mediated immunosuppression by ICA.Methods: Cell proliferation and cytotoxici- ty were assayed by MTT assay. mRNA level of TGF?2 and perforin was detected by RT-PCR assay. assay.Expression of TGF?2 and IL- 2R? was analysed by folw cytometry assay. Results: ICA could inhibit the protein and mRNA expression of TGF?2 in PG cells. It could also reverse the supressed cytotoxicity of LAK, CD3AK cells by TGF?2. The IL-2R? expression on LAK and perforin gene transcription and proliferation of CD3AK inhibited by TGF?2 were also partly recovered. Conclusion: The anti-anti-tumor activity of ICA is at least partly due to its capability of inhibiting TGF?2 expression in tumor cells and restoring the cytotoxicity of immunocompetent cells inhibited by TGF?2.
