Induction of Antibody Response by Intramuscular Immunization with HER2/ neu Oncogene Extracellular Domain DNA in Mice
- VernacularTitle:HER2/neu原癌基因胞外区片段DNA克隆及其在小鼠中诱导的抗体应答
- Author:
Yuejian ZHANG
;
Qiuzao HE
;
Huilian QIN
- Publication Type:Journal Article
- Keywords:
HER2/neu oncogene;
extracellurlar domain;
DNA immunization;
abortion
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To induce an immune response by intramuscular immunization with HER2/neu oncogene extracellular do- main (ECD) DNA and observe the effect in vivo by an abortion model in mice.Methods: Human and rat HEH2/neu ECD gene were cloned and inserted into expressive vector pCDNA3. The DNA immunization, expression of HER2/neu ECD, antibody de- tection and abortion in immunized mice were studied. Results: HER2/neu ECD protein expressed on muscle cells in the injected site, specific antibody to HER2/neu was induced by DNA immunization. The average of the offspring per delivery from immu- nized mice was significantly lower than that from control group, and the abortive embryos were found in uterus from immunized mice.Conclusion: The results show that both human and rat HER2/neu ECD gene can induced effective immune response by DNA immunization.
