Induction of Cellular Immune Response by Intramuscular DNA Immunization with HER-2/neu Extracellular Domain Gene and Its Anti-Tumor Effect
- VernacularTitle:HER2/neu胞外区片段基因免疫诱导特异细胞免疫及其抗瘤效应研究
- Author:
Yuejian ZHANG
;
Qiuzao HE
;
Huilian QIN
- Publication Type:Journal Article
- Keywords:
HER2/neu;
extracellular domain;
DNA immunization;
cellular immunity;
anti-tumor effect
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the cellular immune response and the anti-tumor effects induced by intramuscular DNA immu- nization with HER2/neu extracellular domain gene. Methods: HEH2/neu oncogene extracelltilar domain gene was isolated and inserted to pCDNA3 expression vector. The cellular immune response and its anti-tumor effects were analyzed after intramuscular DNA immunization. Results: A specific cytotoxic activity by spleen cells from HER2/neu ECD gene immunized mice was found in vitro. Tumor growth was inhibited after tumor challenge in vivo in immunized mice. Conclusion: Specific cellular immune re- sponse and anti-tumor effect were elicited by HEB2/neu extracellular domain gene immunization.
