Effect of Recombinant BCG Secreting Human IL-2 on Cytotoxicity of Murine Peritoneal Macrophages
- VernacularTitle:重组人白介素2-卡介苗对荷瘤小鼠腹腔巨噬细胞体外杀伤活性的影响
- Author:
Yuping MEI
;
Xing ZENG
;
Yu HUANG
;
Guolai ZHANG
- Publication Type:Journal Article
- Keywords:
recombinant BCG;
macrophages;
melanocarcinoma cell strain;
cytotoxicity
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of recombinant BCG secreting IL-2 (rBCG-IL-2) on peritoneal macrophages of mouse and inquire into the effect of rBCG-IL-2 on immunity of host. Methods: Gene engineering technology, electricity transfer- ence and inoculation tumor cell are adopted; MTT assay is used to determine tumoricidal effect of macrophages. Results: Tumo- ricidal effect of macrophages of rBCG-IL-2 groups was higher than those of the control coups and BCG groups, and the tumorici- dal effect of macrophages could reach a high state in a week. Conclusion: rBCG-IL-2 can raise tumoricidal effect of macrophages, improve immunity of host and is a prospect biological preparation.
