Prokaryotic Expression, Purification and Characterization of Recombinant Human Interleukin-17
- VernacularTitle:重组人白细胞介素17的原核表达、纯化及鉴定
- Author:
Yonghong WANG
;
Jinhong HU
;
Guoyou CHEN
;
Yongfang YUAN
;
Qin SU
;
Xuetao CAO
- Publication Type:Journal Article
- Keywords:
recombinant human interlukin 17;
prokaryotic expression;
renaturation;
purification;
characterization
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To select suitable conditions for prokaryotic expression and purification of rhIL-17. Methods: rhIL-17 was expressed in E. coli host under heat induction. After compared among the expression amounts in different media under different heat induction time, the most suitable conditions was selected. The target protein was present in the form of inclusion body. The precipitate of inclusion was obtained and purified after 6M guanidine solublization or 2% SDS solublization. Results: Either protocol could yield rhIL-17 with high purity and stable activity. The SDS solublization mehthod gives rise to much more higher productivity than the guanidine solublization method. Conclusion: rhIL-17 were expression in E. coli system and purified to homogenicity by SDS solublization methods with high productivity.
