TC-1 Stromal Cells Enhance Retro viral-Mediated Gene Transfer into Murine Marrow Hematopoietic Cells
- VernacularTitle:TC-1基质细胞系促进逆转录病毒介导的小鼠骨髓造血细胞基因转移的实验研究
- Author:
Xiaodan YU
;
Xiaodan LIU
;
Yuajilin LIU
;
Shuangxi ZHANG
;
Sheng ZHOU
;
Xiushen LI
;
Ring LIU
;
Ning MAO
- Publication Type:Journal Article
- Keywords:
bone marrow stromal cells;
hematopoietic cells;
gene transfer
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the optimal gene transfer protocols of hematopoietic cells mediated by retrovirus. Methods: Murine bone marrow cells were infected by co-culture with murine bone marrow stromal cell line TC-1 or retro-virus packaging cells or retrovirus supernatant. Human mdr-1 and enhanced green fluorescent protein (EGFP) were used as report genes. Results: Stromal cells could greatly increase the gene transfer efficiency when compared with that of supernatant transfection. Transduction efficiency was highest when infected BM cells were co-cultured with virus producer cells. Conculsion: It may be clinically feasible in gene therapy to perform retroviral transduction by co-culture of target cells with stromal cells or cell lines.
