Therapeutic Effects of HSVtk/GCV Suicide Gene System on Murine As-cites Tumors
- VernacularTitle:HSVtk/GCV自杀基因系统治疗小鼠腹水瘤的研究
- Author:
Yiping LI
;
Ning SU
;
Hang YAN
- Publication Type:Journal Article
- Keywords:
HSVtk gene;
ganciclovir;
ascites tumor;
leukemia cell
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of HSVtk/GCV system on ascites tumors. Methods: Using the methods of modified XTT assay, animal experiment, transmission electron microscopy (TEM ) and flow cytometric ( FCM) assay. Results: The sensitivity of P388tk cell was about 37-fold than that of their patental P388 cell. Furthermore we found P388tk cells exhibited potent bystander killing. In vivo , the growth of tumors, which were produced by injecting P388tk cells or a mixture of 50% P388tk cells and 50% P388 cells into DBA/2 mice, was inhibited , furthermore mice survival periods were prolonged contrasted to control groups. Conclusion: HSVtk/GCV suicide gene system could effectively killed the HSVtk-gene-positive P388tk cells and nearby HSVtk-gene-negative cells by the bystander effect in vitro and in vivo.
