Reversal of Drug Resistance in Human Ovarian Cancer Cell Lines by c-jun Antisense
- VernacularTitle:c-jun反义核酸消除人体卵巢癌细胞的抗药性研究
- Author:
Xiaoping GAO
;
Weiyue HAN
;
Zhongrong LIU
;
Bogang LI
- Publication Type:Journal Article
- Keywords:
antisense;
human ovarian cancer cells;
resistance
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate reversal of drug resistance in human ovarian cancer cells by c-jun antisense. Methods: A c-jun antisense was applied to treat resistant and sensitive A2780 cell lines, and to observe the expression levels of c-jun, ?-GCS and GSH in two cell lines. Results: c-jun antisense inhibit c-jun gene expression in resistance cell lines . The mRNA level of the key enzyme in GSH synthesis, ?-glutamyl cysteine synthetase, was also reduced. The GSH content in resistant cells was dropped about 75 % . MTT analysis show that the resistant cells IC_(50) to cisplatin was dropped from 40 ?mol/L to 1.0 ?mol/L after a c-jun antisense treatment. No significant effect was observed in senstive cells (0.2 ?mol/L). Conclusion: A c-jun antisense can inhibit its gene expression in cells, and GSH synthesis in resistant cell was also inhibited. The resistant cells could be reversed to the level of sensitive cells.
