Anergy and Characteristics of Human T Cells Induced by Combination with B7-1 mAb and CsA
- VernacularTitle:B7-1单抗和CsA联用诱导人T细胞无能及其特性探讨
- Author:
Zusen FAN
;
Baoli MA
;
Li WANG
- Publication Type:Journal Article
- Keywords:
B7/CD28;
T cell anergy;
biological characteristics;
cytokine gene profile
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
To study the induced condition and characteristics of T cell anergy in vitro. Methods: Anergic Tcell was induced by combination of B7-1 mAb and cyclosporin A (CsA) in vitro, cytokine gene of anergic T cells was detected by RT-PCR. Results: T cell anergy was antigen-specific. The state of T cell anergy can be reversed by PHA, CD3 mAb and PMA plus A23187. IL-2 can prevent the induction of T cell anergy, but it can not reverse the state of un-responsiveness. IL-2 and IFN mRNA can not express in anergic T cells. In contrast, IL-4 and IL-10 mRNA were detectable. Conclusion: T cell anergy can be induced in vitro , cytokine profile of anergic T cells deviated to Th2-like phe-norype.
