Construction of Eukaryotic Expression Vector for Tumor-Associated Gene SNC90 and Transfection into Cancer Cells
- VernacularTitle:肿瘤相关基因SNC90 cDNA真核细胞表达型重组质粒的构建及其转染研究
- Author:
Wei WU
;
Jiang CAO
;
Xinhan CAI
;
Liyi GENG
;
Shu ZHENG
- Publication Type:Journal Article
- Keywords:
tumor-associated gene;
SNC90 gene;
transfection
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct eukaryotic expression vector of tumor-associated gene SNC90 and transfect it into human colorectal cancer cell lines. Methods: A 1.5 kb tumor-associate gene SNC90 full length cDNA was inserted into a mammalian expression vector pREP9 to make recombinant vector pREP9-SNC90, which was then introduced into three kinds of colorectal cancer cell lines, SW1116, COL0205 and SW620, by lipofection or electroporation. The cells resistant to G418 drug were selected. Results: Cells transfected with pREP9-SNC90 showed fewer G418-resistant colonies than those transfected with void vector, the inhibitory rates are 72.2%, 74.2% and 59.7%, respectively. Conclusion : SNC90 may play a negative role in regulating growth of colorectal cancer cell.
