The Therapeutic Effects of Intratumoral Injection with MHC Class Ⅱ Gene on Tumor-Bearing Mice
- VernacularTitle:MHC Ⅱ类基因瘤体内直接注射治疗小鼠肿瘤的研究
- Author:
Yongjun SUI
;
Peixian TANG
;
Chunmei HOU
;
Ning MAO
- Publication Type:Journal Article
- Keywords:
direct gene transfer;
MHC class II gene;
tumor immunotherapy
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Many gene therapy protocols can induce antitumor immunity, however, the ex vivo approach restricts their uses. This sutydy intended to induce antitumor immunity by direct transfer of MHC class Ⅱ gene in vivo. Methods: MHC class Ⅱ gene cDNA was introduced directly into two tumors: P815, (a murine weak immunogenic mas-tocytoma) and B16 (a murine nonirnmunogenic melanoma) to observe the survival rate of the mice. Results: Tumori-genicity of P815 was reduced when MHC class Ⅱ gene was introduced directly into tumors in vivo. Further more, most vaccinated mice could survive after second challenge of P815. Co-injection of MHC class Ⅱ and B7 genes in the B16 also resulted in the tumor grow slowly, while the injection of MHC class Ⅱ gene was not enough to induce effective antitumor responses. Conclusion: The results showed the potential applications of direct transfer of MHC class Ⅱ gene in the treatment of tumor.
