Determination Method of rhTNF-? Bioassay and Mechanism of rhTNF-? on Human Leukmic HL-60 Cells
- VernacularTitle:rhTNF-?生物活性测定方法的改进及其对人白血病早幼粒细胞HL-60作用机理研究
- Author:
Junzhi WANG
;
Kai GAO
;
Chunming RAO
- Publication Type:Journal Article
- Keywords:
rhTNF-a;
bioassay;
c-myc gene;
HL-60 cells
- From:
Chinese Journal of Cancer Biotherapy
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Through the improvement on determination of rhTNF-? bioassay, we established the formal procedure . Methods; We choose L929 cell for rhTNF-? bioassay determination. To identify the influence of rhTNF-? on the growth of HL-60 cells, various concentration and effecting time were studied. Results: The results showed that the inhibition of rhTNF-? on HL-60 cells based on dose and time. Northern Dot Blot and DNA electrophoresis showed c-myc gene was surpressed, and DNA was damaged by rhTNF-?. Conclusion: Autometic calculation is responsible for bioassay of rhTNF-?. rhTNF-? could inhibit the proliferation of HL-60 cells and its mechanism is different from doxirubicin.
