Preliminary Comparison between rRTA : DS27 and ricin: DS27 as Immunotoxins
- VernacularTitle:rRTA:DS27,ricin:DS27两种免疫毒素的初步比较
- Author:
Wuhong PEI
;
Beifen SHEN
;
Yan LI
;
Yonghuai HE
;
Chunning LAI
- Publication Type:Journal Article
- Keywords:
recombinant ricin toxin a chain;
immunotoxin;
targeting drug
- From:
Chinese Journal of Cancer Biotherapy
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Immunotoxin rRTA:DS27, which was prepared by conjugating DS27 with recombinanl ricin a chain, was compared with ricin:DS27 as immunotoxins. Methods: System analysis were performed regard to their cell-specific cytotoxicity, inhibition on the proliferation of hemoapeutic potential cells, immunogold-labelled intracellular routing and effect of NH_(4)Cl on the cytotoxicity. Results: Results showed that rRTA: DS27 got a more specific cytotoxicity and a weaker inhibition on the proliferation of hemoapeutic potential cells than ricin:DS27, NH_(4)Cl could obsolutelyy enhence the cytotoxicity of rRTA:DS27. Conclusion: rRTA:DS27 had more advantages than ricin: DS27 as immunotoxins.
