Construction of cDNA Library of Ovarian Carcinoma
- VernacularTitle:人卵巢癌cDNA表达文库的构建
- Author:
Xiaoren ZHANG
;
Hailiang GE
;
Ying WANG
- Publication Type:Journal Article
- Keywords:
ovarian carcinoma;
cDNA expression library;
PCR
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
We isolated polyA~+ mRNA direcdy from tumor tissue of ovarian carcinoma using Oligotex~(TM) Direct mRNA Kit (QIAGEN) to synthesize the first and second strand cDNA. The ds-cDNA termini were blunted with pfu DNA poly-rnerase. The blunted cDNAs were added EcoR I adaptor, and then were digested by Xho I . Small cDNA molecules(
