The Suppressive Effect of TNF-? on Growth and Cell Cycle of Human Lung Adenocarcinoma Cells
- VernacularTitle:TNF-?对人肺癌细胞系的生长抑制作用和对细胞周期的影响
- Author:
Haiyan ZHANG
;
Hongru MA
;
Lizhen PAN
- Publication Type:Journal Article
- Keywords:
tumor necrosis factor-a;
lunc cancer cell;
cell cycle
- From:
Chinese Journal of Cancer Biotherapy
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In the present investigation, the cytotoxic activity of tumor necrosis factor a was studied on human lung cancer cells in uitro. A wide range of TNF-ct concentration (from 100 to 10000 U/ml) was tested using the MTT assay. Data presented showed the suppressive effects of TNF-? on A549 dose-dependently and time-dependently; Twenty four hours exposure of A549 human cancer cells lo TNF-? shifted cells from G2 + M,S phase to GO + Gl phase as determined by analysis of isolated cell nuclei with an FACScan cell sorter. The results suggest that the mechanism of the eflect of TNF-a is to influence the cell cvcle of A549.
