EFFECT OF MICROENVIRENMENT ON LIPASE-CATALYZED ENANTIOSELECTIVE ESTERIFICATION OF KETOPROFEN
- VernacularTitle:微环境对脂肪酶催化酮基布洛芬立体选择性酯化的影响
- Author:
Wei DU
;
Minhua ZONG
;
Rong YANG
;
Qiong LI
;
Yong GUO
- Publication Type:Journal Article
- Keywords:
Microcnvirement, Lipase, Ketoprofen, Kineticresolutt
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The effect of reaction media cosolvent water activity, temperature and pH on Novozym 435-caulyzed enantioselective esterification of ketoprofen was systematically explored. Novozym 435 showed high catalytic activity and enantioselectivity in cyclohexane; E value increases markedly by addition of toluene to cyclohexane; the optimum temperature and the initial water activity were found to be 30℃ and 0.09 respectively; pH shows little effect on enzymatic reaction wilson the scope studied.
