Apoptosis inducing effect of hypoxia on cultured neonatal rat cardiomyocytes
- VernacularTitle:缺氧对乳鼠心肌细胞凋亡的诱导作用
- Author:
Litong QI
;
Junhua ZHANG
;
Meng GAO
- Publication Type:Journal Article
- Keywords:
Apoptosis Hypoxia Cardiomyocyte
- From:
Chinese Journal of Interventional Cardiology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
To test the apoptosis inducing effect of hypoxia on cardio myocytes Neonatal Wistar rat cardiomyocaytes were cultured under hypoxic condition for 2 hours,12 hours,24 hours,respectively Flow cytometery,TUNEL and morphological method were used to detect the apoptotic myocytes We found that the number of apoptotic cells of each hypoxia group was greater than that of the control group,and it increased as the time of hypoxia went longer The apoptotic cell number of the control group and the hypoxic groups of 2nd,12th and 24th h. were 9 33?0 62%,17 29?0 95%,33 59?2 09% and 76 01?4 60%( P
