Analysis of AFPmRNA in Human Hepatoma, Paratumor Tissues
- VernacularTitle:人肝癌、癌旁组织中AFPmRNA的差异性分析
- Author:
Ping HE
;
Binbin LIU
;
Shenglong YE
- Publication Type:Journal Article
- Keywords:
hepatoma;
AFPmRNA;
RT-PCR
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the RT-PCR method was employed to detect the expression of AFP in mRNA level in tissue samples form 52 patients suffered from hepatocellular carcinoma (HCC) . The results revealed that the positive rate of AFPmRNA was 76.9% in the HCC tumor tissues and 69.4% in the paratumortissues from the HCC patients with severe cirrhosis . Meanwhile, in HCC patients without cirrhosis, the positive rate reached 50% in tumor tissues, but no AFPmRNA expression was found in related paratumor tissues. The study suggested that the AFP protein was specially expressed by hepatoma cells and mutating hepatocytes. The relationships between AFPmRNA and tumoor size, capsule status and tumor metastasis were also demonstrated.
