Adenovirus-Mediated Vascular Targeting Suicide Gene Therapy in vitro
- VernacularTitle:腺病毒介导的肿瘤血管靶向的体外自杀基因治疗研究
- Author:
Lingfei XU
;
Dehua XU
;
Kai GE
- Publication Type:Journal Article
- Keywords:
vascular endothelial cell;
adenoviral vector;
HSV-tk/GCV system;
EC-cd/5-FC system;
suicide gene
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the potential use of suicide gene systems in vascular targeting gene therapy, HSV-tk/GCV and EC-cd/5-FC systems were established on vascular endothelial cells in vitro by adenovirus transduction. Both modified cell lines were highly sensitive to prodrugs, the IC_(50) for GCV was 0.2?mol/L in IGll/Ad-tk cells, and IC_(50) for 5-FC was 20?mol/L in IGll/Ad-cd cells, while the parental endothelial cells were insensitive even at the highest concentrations of prodrugs in the experiment. Mixed cellular assay showed that significant bystander killing effect was exhibited in modified endothelial cells. Also, the apoptosis involved in these prodrug-mediated growth inhibitions has been shown under electron microscopy. These results indicated that both HSV-tk/GCV and EC-cd/5-FC systems could efficiently suppress vascular endothelial cell growth in vitro , suggesting the feasibility of using suicide gene in vascular targeting gene therapy.
