Inhibitive Effects of Antisense Oligonucleotides of c-fos and c-jun on the Proliferation of the CNE-2Z Cells and the Expression of PKC-?
- VernacularTitle:反义c-fos、c-jun对CNE-2Z细胞增殖及PKC-?表达的影响
- Author:
Zhiwei HE
;
Nanyue CHEN
;
Kangrong CAI
- Publication Type:Journal Article
- Keywords:
c-fos/c-jun;
antisense oligonucleotide;
CNE-2Z cell;
protein kinase C-a;
cell proliferation
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The effects of antisense oligonucleotides of c-fos and c-jun on the proliferation of CNE-2Z cells and on the expression of protein kinase C-? (PKC-?) were investigated. With the interpoising of Lipofectin(LP), the CNE-2Z cells were added with antisens oligonucleotides of c-fos and c-jun. MTT method was used to test the cell proliferation and flow cytometry (FCM) to the PKC-a expression. The results showed that the inhibition of antisense oligonucleotides of c-fos and c-jun to CEN-2Z cells was gradually enhanced with its concentration and prolonging time increasing and that its lowest effective concentration of LP was 1.7 X 10~(-6)?g/ml, the lowest effective concentration was of antisense oligonucleotides of c-fos and c-jun 1.7 x 10~(-5)?g/ml, and the most effective time was 20 hours after treatment. The fluoresence of PKC-a and percentage of positive cells in the groups treated by antisense oligonucleotides of c-fos and c-jun decreased significantly. The results indicated that the autisense oligonucleotides of c-fos and c-jun could inhibit the proliferation of CNE-2Z ceUs the expression of PKC-?.
