Establishment of Two Cell Lines with Double Markers and Comparison between Their Abilities for Detecting Replication-Competent Retro viruses
- VernacularTitle:两种双标记细胞系的构建及其检测具复制能力逆转录病毒能力的比较
- Author:
Min XU
;
Jingsong LU
;
Weirong XU
- Publication Type:Journal Article
- Keywords:
marker rescue;
replication-competent retroviruses;
internal ribosomal entry site
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
In this study, we use retroviral vectors, LXSN (containing internal promoter) and GCEN(containing internal ribosomal entry site,IRES), which cany ?galactosidare gene (LacZ) and neomycin phosphotransferase gene (Neo_R) as double markers, to establish two cell lines, LG-3T3 and GG-3T3. The sensitivities of the two cell lines for detecting replication-competent retroviruses(RCR) in marker rescue test were compared. Our results indicated that both cell lines can be used in marker rescue test, the sentivity of GG-3T3 was 100 times higher than that of LG-3T3.
