Purification of a Recombinant Human GST/MCP-1 Fusion Protein and Its Antitumor Effection
- VernacularTitle:重组人GST/MCP-1融合蛋白纯化及其抗肿瘤作用的研究
- Author:
Yi ZHANG
;
Jiabo TENG
;
Qinong YE
- Publication Type:Journal Article
- Keywords:
fusion protein;
purification;
antitumor
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
GST/MCP-1 fusion protein was overexpressed in Escherichia coli as inculstion bodies. After isolation of the inclusion bodies, the optimum conditions of denaturation and renaturation were studied. The renatured produts were purified to be electroporetically pure by affinity chromatography on immobilised glutathione. The purified product remains biological activities and can react specifically with MCP-1 antibodies by western blot analysis. The in vitro antitumor effect showed that GST/MCP-1 could activate monocytes and lymphocytes to inhibit the growth of a lung adenocarcinoma cell line A549. In vivo, GST/MCP-1 could inhibit the tumor growth in nude mice. These results suggested that MCP-1 had antitumor effect.
